Process_ReplicationInitiation – Replication Initiation

Name
WID Process_ReplicationInitiation
Name Replication Initiation
 
Implementation
Initialization order 10 View in model
 
Reactions
Chemical reactions View in model
Complex formation reactions View in model
 
Parameters
Parameters View in model
 
Comments
Comments Replication initiates once DnaA accumulates at the replication origin, exposing a single-stranded DNA template to DNA polymerase. First, 7 DnaA molecules cooperatively accumulate at each of 4 high affinity boxes near the replication origin. Second, DnaA disrupts the double helix structure of DNA, exposing a region of single-stranded DNA. Third, two DNA polymerases bind the single-stranded region, and begin bidirectional leading strand replication. Next, lagging strand synthesis begins, and lagging strand Okazaki fragments are ligated by DNA ligase. Finally, replication completes when both polymerases reach the replication terminus. Replication initiation is modeled as the stochastic binding and release of DnaA molecules to the genome's 2297 DnaA boxes. DNA polymerization is modeled similar to transcription and translation; nucleotides and energy are distributed among actively polymerizing leading and lagging strands. DNA ligation proceeds subject to the availability of DNA ligase and its cofactor NAD.
 
Metadata
Created 2012-10-01 15:07:35
Last updated 2012-10-01 15:14:00