NadE – NAD synthase (nh3)

Name
WID NadE View in model
Name NAD synthase (nh3) View in model
Cross references EC: 6.3.1.5, BiGG: NADS1, KEGG: R00189
 
Classification
Type chemical View in model
 
Reaction
Stoichiometry [c]: ATP + NAAD + NH3AMP + (3) H + NAD + PPI View in model
 
Catalysis
Enzyme MG_383_DIMER [c] View in model
Optimal pH
8.5 (dimensionless)
Optimal temperature
49.0 (C)
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
ΔG (pH 7.5, 25C, I = 0; kJ mol‑1) -3.10
Keq
172.255658909 ()
 
Kinetics
Forward kinetics
v = Vmax * [NAAD] * [ATP] / (Km1 * Km2 + Km1 * [ATP] + Km2 * [NAAD]); Km1 = 110 (nM), Km2 = 200 (nM), Vmax = 6.9 U/mg (Show evidence)
View in model
 
Associations
Pathways COFACTOR_PROSTHETIC_BIOSYNTHESIS
Process Process_Metabolism View in model
 
Comments
References
  1. Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181

  2. Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

 
Metadata
Created 2012-10-01 15:08:52
Last updated 2012-10-01 15:17:25