NER_DNAIncision – DNA incision

Name
WID NER_DNAIncision View in model
Name DNA incision View in model
Cross references EC: 4.2.99.18
 
Classification
Type nucleotide_excision_repair View in model
 
Reaction
Stoichiometry [c]: ATP + (2) dRibose5P_dRibose5P + (3) H2OADP + (4) DR5P + (3) H + PI View in model
 
Catalysis
Enzyme MG_073_206_421_TETRAMER [c] View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
 
Kinetics
Forward kinetics Vmax = 0.5 1/min View in model
 
Associations
Process Process_DNARepair View in model
 
Comments
Comments UvrA, UvrB, and UvrC form complexes that recognize DNA damage, and incise DNA 3' and 5' to damaged lesions in nucleotide excision repair. UvrA-C function according to the scheme below [PUB_0461]: UvrA2B complex recognizes DNA damage UvrA hydrolyzes ATP and dissociates from UvrB, leaving a DNA-UvrB complex UvrC complexes with UvrB, inducing a conformational change in UvrB which nicks the DNA 4 nt 3' to the damage UvrC nicks the DNA 7 nt 5' to the damage Kinetics Sancar and others report kinetic rates in the range kcat=0.08-0.50 1/min [PUB_0767]. Incision catalyzed by UvrABC at rate 0.39 fmol/min by 20 μmol solution containing 10 nM UvrA, 1000 nM UvrB, and 5 nM UvcR [PUB_0515].
References
  1. Friedberg EC, Walker GC, Siede W, Wood RD, Schultz RA, Ellenberger T. DNA repair and mutagenesis. ASM Press (2006). WholeCell: PUB_0461, ISBN: 9781555813192

  2. Husain I, Van Houten B, Thomas DC, Abdel-Monem M, Sancar A. Effect of DNA polymerase I and DNA helicase II on the turnover rate of UvrABC excision nuclease. Proc Natl Acad Sci U S A 82, 6774-8 (1985). WholeCell: PUB_0767, PubMed: 2931721

  3. Zou Y, Liu TM, Geacintov NE, Van Houten B. Interaction of the UvrABC nuclease system with a DNA duplex containing a single stereoisomer of dG-(+)- or dG-(-)-anti-BPDE. Biochemistry 34, 13582-93 (1995). WholeCell: PUB_0515, PubMed: 7577947

 
Metadata
Created 2012-10-01 15:08:53
Last updated 2012-10-01 15:17:26