MG_447 – multidrug efflux pump

Name
WID MG_447 View in model
Name multidrug efflux pump View in model
Symbol norM
Protein product MG_447_MONOMER
Cross references GenBank: AAC72467.1, CMR: MG_447, BioCyc: MG_447, SwissProt: P47685
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 548303554947 MG_443 MG_444 MG_445 MG_447 MG_448 MG_449 MG_450 MG_451 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 550803 (nt), Length: 1644 (nt), Direction: Reverse, G/C content: 26.8%, Sequence:
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GTGAACAGTAAAAGGGATCGTTTTGAAAAACAACTCCTAATTAAAAATGT
TTTTGAATCAAAACAGCTTTTTCTAACTATTCTTAGATTTACTGTTCCAA
CTTTTTTCTTTGCACTTTTTAGTGCTGCTTATGTATTTATAGATCAAATC
ATGGTGATTAAGTTTGTTCCTAGAAGTGAACTTAATCCTGATTCTATTTT
TACTGATCAGAGTTTAATTGATGAATTTAAAAATTCTGCTTTTTATAAAA
GTGATAGTTTTTTAAGCTCTGGACTTAATATTAAACAATTTATTAAAACT
GTTTTAAATATTAGTCAACCTTTGATTGTTTTGTTAAACGCCATTAATAT
TTTTATTCCTCTAGGAACTGGAGTTATTTTTTCTAAAGCAATTGGTAGAA
ATGATCAAAATAAAATTCAGGAAGCTTGAAATACCGGATTAATATCAACA
ACTGTATTTGGACTAATTACCCAATTTTTGGTTTTATCTTTTGCAAAAGA
ATGGTTGCATTATAATCTTGATCAATCAAGTTTTGAACAAAATTTTCAAG
CTAATAGCTTCCAACAGTTTTTTAACAAAAAGGCTATTGATGTAGCATCT
GAATATGTTTATATTTTGATTGGTCTTAACATCATTCCTATGCTTTCAAG
GTTGTTTTTTTATTTAGCACAATCAGAGGGAAGACAACTTTTTATTGCAA
TTGTTCCGCCTATTGCTAATTTGATTAACATATTAATAGTTTTTTTATTA
GTTAGATATTCTTCTTTAGGAGTAATAGGATCAGCTGTGGCTGGAATACT
AGGATACTTAATTAATTTTTTAGCTTATATTATTTATCTAATTTATTTAA
ACAAAAGAAATCTTACTTATTTAACTTTTAAAACTATTAAATTAAACAAA
ATTGACTTTAATCTACTAGTTGTAGTTTCATTAATAGGAATGGCATCCTT
TTTTAGAAATGGATCACTTTCTATTGTTACTACATTTTATGAGAGTTTTT
TGGTTAATTTGACAAAAGCAACTACTGATAAAAATGATGTTTTTTATCTG
ACACTTTTAACTGGACCTATTGCTATTAGTAATTTAGCTTCAGCAGCTAT
TTTTGGTTTATTACAAGGAGTTAGAACAGTTTCATCATATAAGTTTGGAC
AAAAAAAATATGATGAAATTAAAAAAATTAACATTTATACAGTTATTATC
TGTATATCGTTCGGTAGTTTAATCTATTTGTTAACAGCAGTTGCTTTTGG
AAAACAGATCTTATCTAGTTTTTTTGATGTTTCAGATCAAAATTTGGATT
TAGCAAATTACTTTTCATTAATAGTGCAAGCACAAGTTTTCTTTGTAGCC
ACAGGCGCTAATAGTCAACAATATTTTCAAAATACTAACAGAGTGCTTTA
TTCTTGAATTGTCTCTTTAACACACGGATTGTTTGTATTTATTCCTTTAC
TTTTTATTTTTCAAGCAATAACACTTCAAACAAATAACATTGAAGTTTTT
ATTTGATTGTTAACTGCAAATGCTGCCTTAGCAGGTTTAATTAATATTGC
TTTTGGTCAAATTCACACTAATCTGTTTATGGATAAATATTTCGCTAATC
CACCACAAAATAAGTTGGTCAAATTTATTGAAAAGTACTCATAA
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1644
View in model
Transcription unit TU_318 View in model
Empirical formula (pH 7.5) H17418C15773N6219O11639P1644
Molecular weight (pH 7.5; Da) 531245.98 View in model
 
Functional genomics
Is essential
No (Show evidence)
View in model
Relative expression
0.560583 (dimensionless) (Show evidence)
View in model
Half life
4.425 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
16980200
pI 4.69
 
Comments
Comments Na+-driven multidrug efflux pump [PUB_0110]; mediate resistance to a range of cationic dyes, aminoglycosides and fluoroquinolones [PUB_0240, PUB_0241];
References
  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Brown MH, Paulsen IT, Skurray RA. The multidrug efflux protein NorM is a prototype of a new family of transporters. Mol Microbiol 31, 394-5 (1999). WholeCell: PUB_0240, PubMed: 9987140

  3. Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/

  4. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  5. Kuroda T, Tsuchiya T. Multidrug efflux transporters in the MATE family. Biochim Biophys Acta 1794, 763-8 (2009). WholeCell: PUB_0241, PubMed: 19100867

  6. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:29
Last updated 2012-10-01 15:13:44