MG_407_MONOMER – enolase

Name
WID MG_407_MONOMER View in model
Name enolase View in model
Cross references PDB: 2FYM
 
Genetics
Gene MG_407 View in model
 
Structure
Sequence
1
51
101
151
201
251
301
351
401
451
MGSSNLNINSKITDIFAYQVFDSRGVPTVACVVKLASGHVGEAMVPSGAS
TGEKEAIELRDNDPKNYFGKGVNEAVDNVNKVIAPKLIGLNAFDQLTVDQ
AMIKLDNTPNKAKLGANAILSVSLAVSKAAAKAQNSSLFQYISNKLIGLN
TTNFVLPVPMLNVINGGAHADNYIDFQEFMIMPLGAKKMHEALKMASETF
HALQNLLKKRGLNTNKGDEGGFAPNLKLAEDALDIMVEAIKLAGYKPWDD
IAIAIDVAASEFYDEDKKLYVFKKGIKANILNAKDWSLTSKEMIAYLEKL
TKKYPIISIEDGLSENDWEGMNQLTKTIGSHIQIVGDDTYCTNAELAKKG
VAQNTTNSILIKLNQIGSISETIQTIEVAKKANWSQVISHRSGETEDTTI
ADLAVAAQTGQIKTGSMSRSERIAKYNRLLYIEIELGDKGKYLGWNTFTN
IKPKNFNI*
50
100
150
200
250
300
350
400
450
459
View in model
Is N‑terminal methionine cleaved
Yes
View in model
Prosthetic groups View in model
Empirical formula (pH 7.5) H3571C2228N592O683S14
Molecular weight (pH 7.5; Da) 50027.52 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
47120
Instability index 31.32
Is stable True
Aliphatic index 94.64
GRAVY (25C, pH 7.0) -0.196
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200 View in model
 
Synthesis
Localization c View in model
Chaperones MG_305_MONOMER, MG_392_393_21MER View in model
 
Function
Complex subunit enolase
[c]: (2) MG_407_MONOMERMG_407_DIMER
View in model
 
Comments
Comments Functional Macromolecule Functional as homodimer. Coenzymes Monomer interacts with MG with unknown stoichiometry as prosthetic group [96]. Stable modifications Phosphorylation observed by mass-spec [PUB_0094]. N-terminal methionine cleavage Shewanella oneidensis MR-1 homolog undergoes N-terminal methionine cleavage [PUB_0280].
References
  1. Gupta N, Tanner S, Jaitly N, Adkins JN, Lipton M, Edwards R, Romine M, Osterman A, Bafna V, Smith RD, Pevzner PA. Whole proteome analysis of post-translational modifications: applications of mass-spectrometry for proteogenomic annotation. Genome Res 17, 1362-77 (2007). WholeCell: PUB_0280, PubMed: 17690205

  2. Su HC, Hutchison CA 3rd, Giddings MC. Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae. BMC Microbiol 7, 63 (2007). WholeCell: PUB_0094, PubMed: 17605819

 
Metadata
Created 2012-10-01 15:08:02
Last updated 2012-10-01 15:14:48