MG_388 – Putative metalloprotease

Name
WID	MG_388
Name	Putative metalloprotease
Protein product	MG_388_MONOMER
Cross references	GenBank: AAC71615.1, BioCyc: MG_388, CMR: MG_388, SwissProt: P47628
Homologs	M. pneumoniae: MPN_569

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 490699 (nt), Length: 465 (nt), Direction: Reverse, G/C content: 31.2%, Sequence: 1 51 101 151 201 251 301 351 401 451 TTGAAGTCATCTTTTAGCATTAATTCAAGCCGCCTTTTTAAGCGCTTTTT TGGTAAAAATTACCAAGCAGGAGTCGAACTTTGTTTCCAAATTATTAAAA GCAGTTTAAACCTATCGTTTGACCCAGAATTTGCGCTTTTAATTGTTTCC TGTTCAAAAATGAAAAAACTGAACAAACAGTTTTTAAAACGTAGAGGTTG TACTGATGTAATTAGTTTGTGCTATAACGAAACTGAAACCGGTTTTTCAC TAGCAATCGGCGAAATTTTTTTGTGTCCTAAATACATCTTTAAAAAAGCT AAAGAGATTGGTTGTAACAATTGATTTCTTTTAACTAGATGCTTAATACA CGGCATTTTACACCTCTTTGAATTTAATCATGAAGAAAGTGAAGCATTTG AAAGCGTTACTATGTTTTTTCAAGATGCAATTCATGAAGAGATCTTATCA GTATGAAAGTTTTAA 50 100 150 200 250 300 350 400 450 465
Transcription unit	TU_282
Empirical formula (pH 7.5)	H₄₉₅₀C₄₄₈₄N₁₈₂₇O₃₂₆₈P₄₆₅
Molecular weight (pH 7.5; Da)	151124.18

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	4758600
pI	4.23

Comments
Comments	UPF0054 family [PUB_0096, PUB_0110];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/ Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:27
Last updated	2012-10-01 15:13:36


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