MG_305 – chaperone

Name
WID MG_305 View in model
Name chaperone View in model
Symbol dnaK
Protein product MG_305_MONOMER
Cross references GenBank: AAC71527.1, BioCyc: MG_305, CMR: MG_305, SwissProt: P47547
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 372419379207 MG_302 MG_303 MG_304 MG_305 MG_306 MG_307 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 374919 (nt), Length: 1788 (nt), Direction: Reverse, G/C content: 36.9%, Sequence:
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ATGAGTGCAGACAATGGTTTAATTATTGGCATTGACCTTGGAACTACCAA
TTCTTGTGTTTCTGTAATGGAAGGTGGGAGACCTGTTGTATTAGAAAATC
CTGAAGGTAAAAGAACAACACCTTCCATTGTTTCCTATAAAAACAATGAA
ATTATAGTAGGTGATGCTGCTAAAAGACAGATGGTTACAAACCCAAATAC
CATTGTCTCCATCAAGAGGTTGATGGGTACCTCAAATAAAGTAAAAGTCC
AAAATGCTGATGGTACAACTAAGGAATTAAGTCCTGAACAAGTTTCAGCG
CAAATCCTTAGTTATCTTAAGGACTTTGCTGAAAAAAAGATTGGTAAAAA
GATTTCAAGAGCAGTTATTACTGTTCCTGCATACTTTAATGATGCGGAAA
GAAACGCTACTAAAACCGCAGGTAAGATTGCTGGTTTAAATGTTGAAAGG
ATCATTAACGAACCAACTGCTGCTGCTTTAGCTTATGGGATTGATAAAGC
ATCAAGAGAGATGAAAGTCTTGGTTTATGACTTGGGTGGTGGAACTTTTG
ATGTATCTTTACTTGACATTGCAGAAGGTACTTTTGAAGTACTTGCAACT
GCTGGGGACAACCGTTTGGGAGGTGATGATTGGGATAACAAGATCATTGA
ATATATCTCAGCCTACATTGCCAAAGAACACCAGGGTTTAAACTTATCAA
AAGATAAGATGGCAATGCAACGGCTTAAAGAAGCAGCTGAACGTGCTAAG
ATTGAACTTTCCGCTCAACTTGAAACGATTATTTCTCTACCATTTTTAAC
TGTTACCCAAAAAGGTCCTGTTAACGTTGAGTTAAAACTAACCCGTGCTA
AGTTTGAGGAGTTAACAAAACCACTACTTGAAAGAACAAGAAACCCTATT
TCAGATGTTATCAAGGAAGCTAAGATTAAACCTGAAGAGATTAATGAAAT
TCTTTTAGTTGGTGGTTCTACAAGGATGCCTGCAGTTCAAAAGCTAGTTG
AATCAATGGTACCAGGTAAAAAACCAAACCGTTCTATTAATCCTGATGAA
GTTGTTGCTATTGGCGCTGCTATTCAAGGTGGGGTTTTACGTGGTGATGT
TAAGGATGTTTTACTTTTAGATGTAACTCCATTAACCCTTTCTATTGAAA
CTTTAGGTGGTGTGGCTACTCCTTTAATTAAGAGAAATACAACTATCCCA
GTAAGTAAAAGTCAAATCTTTTCAACTGCTCAAGATAACCAAGAATCAGT
TGATGTGGTTGTATGTCAAGGGGAAAGACCAATGTCTAGAGATAATAAGT
CATTAGGAAGATTTAACTTAGGTGGTATTCAACCAGCACCTAAAGGTAAA
CCCCAAATTGAGATTACCTTTAGTTTGGATGCCAATGGGATCTTAAATGT
TAAAGCTAAGGATTTAACCACGCAAAAGGAAAACAGTATTACCATTAGTG
ACAACGGTAATCTTTCTGAGGAGGAGATCCAAAAGATGATCCGTGATGCT
GAAGCTAACAAGGAACGGGATAACATCATCCGTGAACGTATTGAATTACG
TAATGAAGGGGAAGGTATTGTTAATACCATCAAAGAGATATTAGCAAGTC
CTGATGCTAAGAATTTCCCTAAAGAAGAAAAAGAGAAGTTAGAAAAGCTA
ACAGGTAACATTGATGCTGCTATTAAAGCTAATGACTATGCCAAACTCAA
AGTGGAAATTGAAAACTTTAAGAAGTGAAGAGAAGAGATGGCAAAAAAAT
ATAACCCAACTGGTGAACAAGGTCCACAAGCAAAATAA
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1788
View in model
Transcription unit TU_208 View in model
Empirical formula (pH 7.5) H19173C17384N7452O12381P1788
Molecular weight (pH 7.5; Da) 585966.13 View in model
 
Functional genomics
Is essential
Yes (Show evidence)
View in model
Relative expression
1.96428 (dimensionless) (Show evidence)
View in model
Half life
2.4 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
18455900
pI 3.79
 
Comments
Comments interacts with backbone [PUB_0014]; binds short, linear, unfolded, hydrophobic peptide segments [PUB_0014]; ATP hydrolysis regulated by co-chaperone grpE [PUB_0014]; Several rounds of ATP- dependent interactions between dnaJ, dnaK and grpE are required for fully efficient folding [PUB_0096]; 1 DnaK monomer: 1 DnaJ dimer: 1 GrpE dimer [PUB_0230];
References
  1. Eds Lund P. Molecular Chaperones in the Cell. Oxford University Press, New York (2001). WholeCell: PUB_0014, ISBN: 9780199638673

  2. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  3. Genevaux P, Georgopoulos C, Kelley WL. The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. Mol Microbiol 66, 840-57 (2007). WholeCell: PUB_0230, PubMed: 17919282

  4. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  5. UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/

  6. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:23
Last updated 2012-10-01 15:13:24