MG_295 – tRNA U34 sulfurtransferase

Name
WID MG_295 View in model
Name tRNA U34 sulfurtransferase View in model
Symbol mnmA
Synonyms asuE, trmU
Protein product MG_295_MONOMER
Cross references GenBank: AAC71516.1, CMR: MG_295, BioCyc: MG_295, SwissProt: P47537
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 360420366524 MG_293 MG_294 MG_295 MG_296 MG_297 MG_298 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 362920 (nt), Length: 1104 (nt), Direction: Reverse, G/C content: 28.2%, Sequence:
1
51
101
151
201
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351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
ATGTCAATTATTGCAAAAACAGTTTTTATAGGTTTAAGCGGTGGTGTTGA
TTCTGCTGTTAGTGCTTTACTTTTAAAAAAGCAATACCAAGAAGTTATTG
GTGTTTTTATGGAATGTTGGGATGAGACACTTAATAATGATTTTTATGGT
CATAAGAAAATAAATAATAACAAATCAGGTTGTTCATCTTTTCAAGACTT
CCAACAGGCTAAAAAAATCGCTAATTCTTTAGGAATTAAGTTAATAAAAA
AAAACTTAATTGAAGCTTATTGAAACAAAGTTTTTTTACCTATGATTCAA
AGTTTCAAAAAAGGGTTAACCCCAAATCCAGACATCTGGTGTAATCGTTT
TATTAAGTTTGGTTTATTGCATGATTTTTGTAAGCAAATTAACCCTAATT
CTCTTTTTGCAACTGGTCATTATGCCAAAATAAACATGATAGAAAATCAG
CCTTTGCTTTCTATTCCTAAAGATACCAATAAAGATCAAACTTATTTTTT
AGCAAATGTTAAAAAAGAACAATTTCAGAATGTTATTTTTCCTTTAGCAG
ATTTAAAAAAAATAACAGTGAGAAATATTGCTAGAGAAAATAATTGAGAA
GTTGCAGATAAAAAAGATTCAACTGGAATTTGTTTTATTGGTGAAAGACA
TTTCAGTGATTTTTTAAAAAACTATTTACCTGTAAAAAAAGGATTAATTA
AGGATTGAAAAACCAAACAAACTATTAGTGAACATGATGGTGTTTGGTTT
TATACGATTGGTCAACGCAGTGGATTAAATTTAGGGGGGTTAAAACAACG
TCATTTTGTTGTTGCTAAGGATATTGAAACTAATGAATTATTTGTTTCTT
GTGACAAAGAAGAATTATTGAAAACAACAATTTTATTGGATCAATTTAAC
TGGTTGTATACACCAAAGCAACTTCCTAGTCAAGTTCTGGTAAGAATTAG
ACATGCTCAAAAACCAGAAATTGCAAAGTTGAAATTATTATCAGATAATA
AACTGGAAATAACATTTAAAAATCCTGTTATAAGTGTTGCATCTGGACAG
TTTGGTGTATTATATACACTTGATCAAATTTGTTTAGGAGCAGGATTAAT
TTAA
50
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250
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350
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500
550
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650
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800
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950
1000
1050
1100
1104
View in model
Transcription unit TU_199 View in model
Empirical formula (pH 7.5) H11765C10660N4380O7696P1104
Molecular weight (pH 7.5; Da) 358567.81 View in model
 
Functional genomics
Is essential
Yes (Show evidence)
View in model
Relative expression
1.29325 (dimensionless) (Show evidence)
View in model
Half life
5.1 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
11563300
pI 4.07
 
Comments
Comments Catalyzes 2-thiouridination (s2U34) of wobble position (34) [PUB_0045]; hypermodification increases efficiency of wobble recognition for Lys, Gln, Glu.
References
  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Cabedo H, Macián F, Villarroya M, Escudero JC, Martínez-Vicente M, Knecht E, Armengod ME. The Escherichia coli trmE (mnmE) gene, involved in tRNA modification, codes for an evolutionarily conserved GTPase with unusual biochemical properties. EMBO J 18, 7063-76 (1999). WholeCell: PUB_0045, PubMed: 10601028

  3. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  4. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:23
Last updated 2012-10-01 15:13:22