MG_277 – Protein-export membrane protein secDF

Name
WID MG_277 View in model
Name Protein-export membrane protein secDF View in model
Symbol secDF
Protein product MG_277_MONOMER
Cross references GenBank: AAC71499.1, CMR: MG_277, BioCyc: MG_277, SwissProt: Q49409
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 332912340825 MG_275 MG_276 MG_277 MG_278 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 335412 (nt), Length: 2913 (nt), Direction: Reverse, G/C content: 33.8%, Sequence:
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2251
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2901
GTGAGGTTCAAAAAGCGCTTTTCTTTGGACTGAATCCTAAAAATAGGAAC
TATTCTTGGACTTGTTTGTCTGGGTTTGTTTGGTGTTATTTTTGGGAGTT
ACAAACTGTTAAATGACAGCAGGTTAGGTGCTGTTTTCAACGGTTCTACC
ACTACAACTGTTTACTTTTTAAACCATAAGTCAACTAACAATACCTCATT
AGATCCTCAGCAAACAAAACCAACCAACGGGATTGAAAACATCACTAACA
TTGATAGCTTTTTAGATGGTGTTGAAAAAAGTTATGCTAACAGTTTATTC
ATTCAGGGCTTTTCTTCTGTAAACATTACTAAAAACACTAATGATAAAAC
TGCTAGTGAACTTGACAACATTGACAAGTCCTGATTGGTTAATGGGGGAT
TACCTTCTGTTACTCTCACCTTTGAACAAAACCGTGAGCAAGCCAAAACC
CGCCAAAGAAAAAGACAAGTTGATGCTCAGGTTAGAAGAAATGCAATTAG
TTCAGTTGAACACAACTACCAACTTTCACTTGAAACAACTGATGGAGTGG
TTTTATTTGATTCACTGGATAATAATTTTATTAATGCTTCCATTAGGGCA
GTTGTTCCCCAAAACACTTCTGTAAACAGTGCTTTAACCTTTGAATACAA
GTTAAATAAAAATGTAGTTACCAAAGAAAGTTTACACACCGATTTTCTTG
ATTTTATCAACTCTAAAAACTTATCAAGTTCTGATTATAATACTGGTAAT
GGGCAAGCTAGTGTTGAGGGCAATGGTAAATTTTTTAAGCAAAATGCGAA
TGGAACTTCAAGTAGTGGTAATAAAACTTTAGTATTGTGAAAAGATAAAC
AAGGCGCATTAAACTATGTCCGTAATATCTTTAATGTAGTTGAGGGTAGT
AGTGATTATCTCACCTTTAATGAAAGAGAGAAGAGTCTCTGGGAATTTTT
ACATGCAAAGGGTAGCTTTGCTAGTGGTGATAATCTGTTTTTAAACACTG
ATGCTAATGGGGCATCTCCTATTAAAAAAGCAAGTGACATTACTCTCAAA
AACCTTTATTATATCTACGCTGCTCCTAACCACTTTTCAGCGGTTGCTAG
CAACAGTAATGACAATAATAACCAGAATAATAACAATAACAACAATAGTT
CAGATGTAATTACAGTTTCAAATAGTGCTGATACAAGAAAGTTAAGGAGT
GCAGATAGCAGTGGTTTTTCAGGGTTATTCCATAACTATATTATCAGTGA
AATTAGAACTGAAGATCCGGTTAGCAAAACCGGTGATCCTGTTAAGATTA
ATCCTACTCTACAAAGCTTTTTAGACACTAACAACCAACGGATAGAATAT
GGTGGTAATATTAAGTTTCAAGTTGGTAATTTCTTGAGTAGTGATGGTAC
ATACACCCCACCAAACTTTGTAACAGCAGCTACTGTTAAAGAGCTTTTAA
CAAATCCATTTCCCACAGCAGCTACCATTGCAACTGCAAAAACAAGTCTG
GTTAACGCTCCACTTGCAAATACCATCACAGATGTTTCTAATTTTGCTTC
TAGTTTTATTGCGTTTGGTATCATCGTTCTTATTGCTGCTGTTCTATTAA
CACTACGATACAAGCTCTTAGGGTTATACAAAGCATTAGCTTTAGGGTTA
AGTGTGGTGTCATCACTGGTTATCTTTAGTGCAGTTGGTGGAGTTGTTGA
TGTCTTTTCTTTTGTGGGCATCTTCTTTGTTATTGCCATCAACCTAATTA
ATCTCTTGAATTTAGGAGAGTTATTCTTAAGAAATATTAGAAATAATGCT
TCTATTATCGAATCGTGAAAACTGTGTTTAAAAAGAAGCTTTTTTGCTAA
TCTTGAGTTTCACATTACCTGGTTAATTAGTGCTTTAGTTGTTATCTATC
TTTCTAATTATCAGGTCCAACAACTAGCTAATTTGATGGCAATATCAGCC
ATTACTTCTTACTTTTTTAGTTATGGAATCAGCATCGTTTTAATCTCACT
GTTTGTCAGTTCAGAATCAGGTGCAAACTGAAAGCTTTTTCTCTATCAGA
AGGATGCAAAAGCACTAACTAAAACTTCTAGTAACTATTCACTTTTAAGT
TCAACTAGTGACTTGAACACAGACTTTTTTATAACCAAAAACCAACATGA
TTTCTTTTTGAAAAACAACTGGAAGTTTTTGTTTGTATGGCTAATCTTGT
TAGCTATAGGAGTTGTAATGCTAGTTTTATATCTTGTTCAACCTAACCTG
TTAGGAGAATTTTTAGCAGCAGATGTTGAATCAAGTAACGGGATTATTGC
TGGGATAGGAATAGTTAGTTTACTGTACTTAGCTTATTGTTTGATTAGAT
ATGGTGTTGCTTATTGTTTAAGTTATTTGGTTAGCTTTATCTTGTTATGT
AGTGGTTTGTTTGCAGTGATGTATCTAACTAACTATCTGTTTAGTATAGA
CCAATCAACAATCCAGTTAATTACCTTTGTTTATCTGTTTTGGCTCTTTT
TTGCAGCTAAGGTATCACAAACAACAACTTGGACTTACTTTTACTGGTTT
AAAAGGAGCTTAAAGGACAAGGTATTTATCAAAAACTTATTTAACGATAA
CTTCAACTCGCAGTGGAAGATAGATCTGATTGAAAGTTCTAGCTTAGTTT
TCATTTTTATTATCTACAGTGGTTTTAACTTTGGTGGGATTGATGGCAAC
TTTAACCTTGTGATCTTTTATCTAATTGCTATTGTTGGTTTATTTGATGT
TGCAACTGCTTTTTTACCAATGTTTTGCTTTGGTTTAATCAATGGTTGAC
TCAGTCCTTTCAACTATGTTCATTCCCGTTTGAGCTTAAAACACAAAAAA
CATCCTTTTAAAGAGATTGATCAGATTGAAGAGCAGTTAATTGCAGGAAT
TAACAGTTTTTAA
50
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2913
View in model
Transcription unit TU_184 View in model
Empirical formula (pH 7.5) H31020C28106N11493O20512P2913
Molecular weight (pH 7.5; Da) 948223.17 View in model
 
Functional genomics
Is essential
Yes (Show evidence)
View in model
Relative expression
2.98349 (dimensionless) (Show evidence)
View in model
Half life
4.425 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
29732400
pI 4.27
 
Comments
Comments homologous to secDF of M. pneumoniae, B. subtilis [PUB_0169]; secDF increases efficiency of translocase; annotated as gerBB in 1995 Science annotation [PUB_0057]; however mycoplasmas don't form spores [PUB_0168]; gerB is receptor for nutrient-triggered germination [PUB_0167]; together with gerK responds to l-asparagine, glucose, fructose, and K+ [PUB_0167]; triggers release of DPA which triggers enzymes which initiate hydrolysis of spore's peptidoglycan cortex leading to completion of germination [PUB_0167]; also transduces pressure to trigger germination [PUB_0167]; interacts 1:1 with secYEG [PUB_0328];
References
  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Black EP, Koziol-Dube K, Guan D, Wei J, Setlow B, Cortezzo DE, Hoover DG, Setlow P. Factors influencing germination of Bacillus subtilis spores via activation of nutrient receptors by high pressure. Appl Environ Microbiol 71, 5879-87 (2005). WholeCell: PUB_0167, PubMed: 16204500

  3. Dandekar T, Huynen M, Regula JT, Ueberle B, Zimmermann CU, Andrade MA, Doerks T, Sánchez-Pulido L, Snel B, Suyama M, Yuan YP, Herrmann R, Bork P. Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames. Nucleic Acids Res 28, 3278-88 (2000). WholeCell: PUB_0169, PubMed: 10954595

  4. Davidsen T, Beck E, Ganapathy A, Montgomery R, Zafar N, Yang Q, Madupu R, Goetz P, Galinsky K, White O, Sutton G. The comprehensive microbial resource. Nucleic Acids Res 38, D340-5 (2010). WholeCell: PUB_0168, PubMed: 19892825, URL: http://cmr.jcvi.org/

  5. Duong F, Wickner W. Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme. EMBO J 16, 2756-68 (1997). WholeCell: PUB_0328, PubMed: 9184221

  6. ... 3 more

  7. Fraser CM, Gocayne JD, White O, Adams MD, Clayton RA, Fleischmann RD, Bult CJ, Kerlavage AR, Sutton G, Kelley JM, Fritchman RD, Weidman JF, Small KV, Sandusky M, Fuhrmann J, Nguyen D, Utterback TR, Saudek DM, Phillips CA, Merrick JM, Tomb JF, Dougherty BA, Bott KF, Hu PC, Lucier TS, Peterson SN, Smith HO, Hutchison CA 3rd, Venter JC. The minimal gene complement of Mycoplasma genitalium. Science 270, 397-403 (1995). WholeCell: PUB_0057, PubMed: 7569993

  8. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  9. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:22
Last updated 2012-10-01 15:13:20