MG_254 – DNA ligase, NAD-dependent

Name
WID MG_254 View in model
Name DNA ligase, NAD-dependent View in model
Symbol ligA
Protein product MG_254_MONOMER
Cross references GenBank: AAC71474.1, CMR: MG_254, BioCyc: MG_254, SwissProt: P47496
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 300346307326 MG_251 MG_252 MG_253 MG_254 MG_255 MG_256 MG_494 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 302846 (nt), Length: 1980 (nt), Direction: Reverse, G/C content: 30.4%, Sequence:
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GTGATGGATGTGAAATTGAAGATTCAACAGCTGGTTAACTTAATAAAAAA
CTATGACTATCACTACTATGTTTTAAGCGAACCTTTAATTGATGATTTTG
AGTATGATATGTTGTATAAGTCACTCCAACAATTAGAAAAAGATCATCCT
GATTTAATCCAAATTGATTCCCCTACCCAAAGGGTGGGAGGAGAAGCTGT
GAAGGGTTTTAAAAAGTTAAACCATAACAGTCCAATGCTCTCTTTGGAAA
ATGCTTTTTCAACTAAAGAAATTGCTAATTTTATTGATAATATTAACTTT
CAAACAAACTCAAAAAATGAATTTGTAGTTGAACCTAAAATTGATGGAGT
TAGTATCTCTCTAACTTATAAAAATGGTGTTTTAGTTCATGCTTTAACCA
GAGGAGATGGAAGTGTTGGGGAAGATGTTTTAAATAATGTTAAAACCATT
AAATCTATCCCTTTAACAATCCCTTTCACAAAAACAATTGAGATTAGGGG
TGAGATTTTTGTTGATAAAAAAACTTTTTTAGCAATTAACAATCAACTTG
AAAAACCATTTGCTAATGCAAGGAATCTAGCAGCAGGTACAATACGTAAT
TTAAACAGTGAAATCACTGCACAGCGCAAATTAAGGGCATTATTTTATTA
CATCCCTAATGGTTTGGAAGAGTCAATCACTACTCAAACTATGGTTTTAG
AACAGCTTAAGCAGTGAAAATTCCCAGTTAGTGATACCATCAGGGTTTTT
CAAAACAAATTTCAATTAATTAATTACTTGGAAGCGTTTGACAAAAAACG
AGAACAGTTAACTTTTAATCTTGATGGTTTAGTTATTAAACTAAACAGCT
TGCTTTTTTATCAACAATTAGGTGCTACAAGTAAATCACCACGTTGGGCA
ATAGCATTTAAATTTAGTCCTAAATTTGTTCAAACTAAATTAACAGCAGT
TCTTATAACGATTGGTAGAACTGGTAGAGTGAACTATACTGCTAAATTAG
AAAGTGTTAATTTAGATGGAACAAAAGTAACAGCTGCTACTTTACATAAC
TTTGATTACATTAAAACTAAAGACATTAGGATCAATGACACTGTTGTTAT
CTATAAAGCTGGGGAAATTATCCCTAAAGTACTAAAGGTAAATCTTGAAA
AAAGAAAAAATGACACTATCATAATTCAAGAGCAAAAATATTGTCCTTCA
TGTAATTCAAAACTAGTCAAAATAGTTGATGAAGTTGATCAGTATTGTAC
CAATGAAACTTGTAAGGAGCGAAACATCCAGTTAATTAACTATTTTGTTT
CTAAAACTGCTATGGACATTAACGGGTTGAATATTAATACTATTACCAAA
CTTTATGAACACAATTTGGTTAGATCTATAGTTGATCTTTATGATTTAAA
AGACAAGAAAAACCAAGTTTTAAAATTAGATCTGAAGATTGGTGATAAAC
TTTTCAACAAGTTAGTTGATAACATTGAAAATTCAAAACAAAAAGGAATG
GCTAGATTACTAACAGGACTTGGTATTAAGCATGTTGGTAATGTATTAGC
TAAGAATTTAGCTAATCATTTTAAAAATATCAAAGCATTACAGCATGCTA
GCTTAGAGAACTTAATTAGTTTAAATGATGTAGGAATAACAGTAGCTGAA
TCATTGTATAACTGGTTTCATGACCCTAACCATTTGCAGTTAATTGAACA
ACTTGAATTAAGACAAGTAAAAACAGATCAATTACCACTGAAAATTAACT
TTGAAACTAACAGTATTTATTTTCAAAAACGCTTTCTTATTACCGGTAGC
TTTAACATTAGTCGTGACCAAATTAAGGATTTATTATCAGCTAAGTTTGA
TTGCCAGTTTGCAAGTGAAGTCAAACCAACAGTTGACTTTGTTATTGCAG
GAAACAAACCAACTTTAAGAAAAATCAATCACGCCAAAGAACTGAACATT
CCTATCATTAATGAAGCAATTTGAACATAG
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View in model
Transcription unit TU_165 View in model
Empirical formula (pH 7.5) H21156C19175N8025O13732P1980
Molecular weight (pH 7.5; Da) 645063.96 View in model
 
Functional genomics
Is essential
Yes (Show evidence)
View in model
Relative expression
0.369079 (dimensionless) (Show evidence)
View in model
Half life
6.1 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
20667300
pI 3.88
 
Comments
References
  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  3. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:21
Last updated 2012-10-01 15:13:16