MG_250 – DNA primase

Name
WID MG_250 View in model
Name DNA primase View in model
Symbol dnaG
Protein product MG_250_MONOMER
Cross references GenBank: AAC71470.1, BioCyc: MG_250, CMR: MG_250, SwissProt: P47492
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 295151301975 MG_248 MG_249 MG_250 MG_251 MG_252 MG_253 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 297651 (nt), Length: 1824 (nt), Direction: Reverse, G/C content: 29.6%, Sequence:
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1801
ATGGTCAATAAAAGCAACAGTTTAGATGAACTTTTAAAGCAGATTAAAAT
TACTGAAATTATTCAACACTACGGGGTTAAAATCCAAACTAAGGGTAATA
GTCTACTTGCTTTATGTCCTTTTCATGATGATAAAAATCCTTCTATGTCC
ATTTCCAGTTCTAAAAACATCTTTAAGTGTTGGGCTTGTAATGCAGCTGG
CAACGGAATAGCGTTTATCCAAAAGCATGACCAGTTAGATTGAAAAACTG
CACTTAAAAAAGCAATTGAAATTTGTGGAATTAAGTTAGAAAATTGGAAC
AGTAATTTACTAACAAAAGTTGATCCAAAACAAAAACGATATTGGGAGAT
AAACAATGCTTTAATTACTTATTATCAAACCAGATTAAAAAGAGAAACAA
ACCCAAATGGGATGAATTATTTAGTTGAAAAAAGAAAGCTTAATAAAACA
TTAATTGAACAGTTTCAGCTAGGACTTGCTTTTCACAATGAAGATAAGTA
TCTATGTGAAAGTATGGAAAGATACCCCTTCATTAATCCAAAGATAAAAC
CGAGTGAATTGTATCTTTTTTCAAAAACTAACCAGCAAGGTCTTGGCTTT
TTTGACTTTAATACCAAAAAAGCTACCTTTCAAAACCAGATTATGATCCC
TATCCATGACTTTAATGGTAACCCGGTTGGTTTTTCAGCAAGAAGTGTTG
ATAACATCAACAAACTGAAATATAAAAATAGTGCTGATCACGAATTTTTT
AAAAAAGGGGAGCTGTTATTTAACTTTCACAGGTTAAATAAAAACCTCAA
TCAACTCTTTATTGTGGAAGGTTATTTTGATGTTTTTACACTAACAAACT
CCAAGTTTGAAGCTGTTGCATTAATGGGATTAGCATTAAATGATGTGCAA
ATTAAAGCCATTAAAGCTCACTTTAAGGAGTTACAAACCTTAGTTTTAGC
ACTTGATAATGATGCTAGTGGTCAAAATGCTGTGTTTAGCTTAATTGAAA
AACTTAATAACAACAATTTTATTGTGGAAATTGTTCAGTGAGAACACAAC
TATAAAGATTGGGATGAACTGTATTTAAACAAGGGTAGTGAGCAAGTTAT
ATTACAAGCAAACAAAAGACAAAATCTAATTGAATATCTTGTTAGTTTTT
TTAAAAAGCAACAACTTGATCAAAGGGTTATTACTAATAAAATCATTGCT
TTTTTAACAAAAAACCAAACAATTTTAAACGACCATAGTTTTTTAATTTT
TCTCATTAAAAATTTGGTTAAACTACTTGAATATAGTGATGAAAAAACTC
TGTATGAAACAGTTTTAAAACACAAAGAAAAACTTGTATCTAAGTTTGAT
AACAACCGTTTTTACATAAATACTTCAGGCCATGCTCAACCACCACAAGA
ATTGCAAAAAACCACTGCAGCACTAGTGCAAACAGCTTTTGAAGAAGCAG
TTAATGAGTTGTGAAAACCTGAAATCTTTGCGTTTGCTTTAATTGATAAA
CGCTTTTTGGTTGAATTAAAACAATCCCATCTAGATGAAGTTTTTAAGGA
ATGTAACTTTAATTTGTTTGATGTTGAACTTTTTATTGAAAAAGCAAGGA
TCTATTGGAGTGAAAATCAAACTGCTAACTGAGTTGGTTTTGAAAGTGTT
TTAGATCAAAATTACCTTTTAAACAATAAAGCAAGGTTATTGGAAATTAA
AGATATTTTTTTAGATGAATTAACTTGTTATCAAGCTAATGATTTTCAAA
ACTATCTAAAGACCTTTCAAACGTTATTAAAACAACAAAAGCAGCGCTTA
AAAAATTTAAAGTTAACGCTATAA
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1824
View in model
Transcription unit TU_164 View in model
Empirical formula (pH 7.5) H19486C17661N7383O12643P1824
Molecular weight (pH 7.5; Da) 593949.00 View in model
 
Functional genomics
Is essential
Yes (Show evidence)
View in model
Relative expression
0.369079 (dimensionless) (Show evidence)
View in model
Half life
2.2 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
19011500
pI 3.88
 
Comments
Comments 3 primase bind each dnaB helicase [PUB_0201];
References
  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  3. Mitkova AV, Khopde SM, Biswas SB. Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis. J Biol Chem 278, 52253-61 (2003). WholeCell: PUB_0201, PubMed: 14557266

  4. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:21
Last updated 2012-10-01 15:13:15