MG_209_MONOMER – 23S rRNA pseudouridine synthase; U955, U2504, U2580

Name
WID MG_209_MONOMER View in model
Name 23S rRNA pseudouridine synthase; U955, U2504, U2580 View in model
 
Genetics
Gene MG_209 View in model
 
Structure
Sequence
1
51
101
151
201
251
301
MKQCFVVTTTKRLDSLLASLLNLSRVKVVKLIMNGQIKVNEKLTFKNSLI
VAKDDVIKVEIHDETTSDFITSVEPYNLKLEVLFEDKDLMVINKPSGLLT
HPTTFNEKASLLAACIFHNNKNPVYLVHRLDRDTSGAIVVCKNQASLLNL
QNQLQNRTLKRYYVALVHFPFNALTGSINAPLARVNNNKVMFKIAQTAKA
KQAITKFKVINQNEKAALISLELLTGRTHQIRVHLKFIQHPVYNDPLYGI
KSEKKDSYGQFLHANRICFIHPTLNKPMDFHAPLEPKFSTKLKSLNLSLT
DPLHVLFK*
50
100
150
200
250
300
309
View in model
Is N‑terminal methionine cleaved
No
View in model
Empirical formula (pH 7.5) H2585C1589N431O438S9
Molecular weight (pH 7.5; Da) 35023.63 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
10930
Instability index 17.64
Is stable True
Aliphatic index 105.66
GRAVY (25C, pH 7.0) -0.122
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200 View in model
 
Synthesis
Localization c View in model
 
Function
Enzyme View in model
 
Comments
Comments Functional Macromolecule Functional as monomer [PUB_0121, PUB_0122].
References
  1. Del Campo M, Ofengand J, Malhotra A. Purification and crystallization of Escherichia coli pseudouridine synthase RluD. Acta Crystallogr D Biol Crystallogr 59, 1871-3 (2003). WholeCell: PUB_0122, PubMed: 14501142

  2. Sivaraman J, Sauvé V, Larocque R, Stura EA, Schrag JD, Cygler M, Matte A. Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP. Nat Struct Biol 9, 353-8 (2002). WholeCell: PUB_0121, PubMed: 11953756

 
Metadata
Created 2012-10-01 15:07:52
Last updated 2012-10-01 15:14:34