MG_203_204_TETRAMER – topoisomerase IV

Name
WID MG_203_204_TETRAMER View in model
Name topoisomerase IV View in model
Cross references PDB: 1S16
 
Structure
Biosynthesis [c]: (2) MG_203_MONOMER + (2) MG_204_MONOMERMG_203_204_TETRAMER View in model
No. subunits 4
DNA footprint Length: 34 (nt), Binding: dsDNA, Region: dsDNA View in model
Empirical formula (pH 7.5) H23222C14432N3822O4234S70
Molecular weight (pH 7.5; Da) 320263.49 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
-194160.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
 
Synthesis
Formation process Process_MacromolecularComplexation View in model
Localization c
 
Regulation
Regulatory rule !ciprofloxacin & !difloxacin & !sparfloxacin View in model
 
Function
Enzyme Negative supercoiling (topoisomerase I)
[c]: (2) ATP + (2) H2O ⇒ (2) ADP + (2) H + (2) PI
View in model
Reaction participant View in model
Complex subunit topoisomerase IV
[c]: (2) MG_203_MONOMER + (2) MG_204_MONOMERMG_203_204_TETRAMER
View in model
 
Parameters
Parameters View in model
 
Comments
Comments Composition [PUB_0006]. DNA-binding Topoisomerase IV has 34 nt footprint [PUB_0694]. Regulation: Fluoroquinolone antibiotics inhibit topoisomerase IV [PUB_0847].
References
  1. Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181

  2. Knox C, Law V, Jewison T, Liu P, Ly S, Frolkis A, Pon A, Banco K, Mak C, Neveu V, Djoumbou Y, Eisner R, Guo AC, Wishart DS. DrugBank 3.0: a comprehensive resource for 'omics' research on drugs. Nucleic Acids Res 39, D1035-41 (2011). WholeCell: PUB_0847, PubMed: 21059682

  3. Peng H, Marians KJ. The interaction of Escherichia coli topoisomerase IV with DNA. J Biol Chem 270, 25286-90 (1995). WholeCell: PUB_0694, PubMed: 7559669

 
Metadata
Created 2012-10-01 15:07:38
Last updated 2012-10-01 15:14:07