MG_183 – oligoendopeptidase F

Name
WID MG_183 View in model
Name oligoendopeptidase F View in model
Symbol pepF
Protein product MG_183_MONOMER
Cross references GenBank: AAC71402.1, BioCyc: MG_183, CMR: MG_183, SwissProt: P47429
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 206067212891 MG_180 MG_181 MG_182 MG_183 MG_184 MG_185 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 208567 (nt), Length: 1824 (nt), Direction: Forward, G/C content: 32.2%, Sequence:
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ATGAAAAATAGTTACAAGTGGGATCTATCAGTTTTATTAAACAACCAAAG
CTTACAAGCAAATTTTTTAAAAATTCAAACAGTTAGTGAAGCGTTAATTA
AAGCTTATAACAACGGGTTGTGTTTTACAAATAAAACTAGCTTTGAACAG
TTTTTAGCAATCGATGATAAGTTCACTGAACTTGAAAATCGTTACACTAA
CTACCTTTATAACAAGCAGAATGAAAATAACTTGGATAAGGAGGTTAATG
ATGCAATTTTTGCATACCAGAGTTTTAAAAATAACCATAACCTTGCTTTC
AGTACACTGCAACAGGAGTTATATAACCATGAAAAACTCATTAAGGATTA
TTTAACTGATCCAAAGCTAGCGGTTTACAAGCGCAACTTAATGTTAGTTT
TTCGCGATAAACCCCACCAACTATCTAGTCAAACCCAGAGTTTATTGAGT
CAAATTAACCCTTGTTTTAACCAAGCAGAACGGATTTTTAACATCCTTTC
AACTGCTGATTTAAACTTGCAACCTGTTGTTTATCAAAACAAAAAATATC
CGATTAACAGTGTGAGTGATTATCAGTCCTTACTTGAAAACACTAACAGG
GGGATTAGAAAAGCTTGTTATGAGAAGTGGATTGAAATTTATTGAACTAA
TAGAAACAGCTTAAGTTTAAGTTTGGTGGAAAATTACATCCAACTAGAGA
ACTTCGCTAAACTAAAGAACCATCCTAGTTACATTGCCCAAACTGCTTTT
AATGATGAGATTGAAGTTGGTTTTATTGATTTTGTTTACCAACAAGTAGC
TCAGTTTGCCAAAACCTTTCAAGCATTTATTCGCTTAAAAAAACAGATTT
ATAAACATGTTTTAAAAGTCAATAAAGTTGAACCATATGATCTTACCCTA
ACACTTTTTAAAACTAAAAAATCATACACGATTGAACAAGCTAAACAAGA
TGCACTAAAAGTTTTAGATCTACTTGGTGACAACTACATCAAGATAGTTA
AAAAGGCTTTTAATGAAAACTGGATTGATTGACTAGCAGATAAAAACAAG
TACACAGGGGCATATTCAATCTCCAATGTTAAGGGCTTAGAGCACTTTTT
TATCTTAATGAACTTTGATAAAACCAAATCATCACTAAATACGTTGGTAC
ATGAACTTGGTCATTCAGTTCACTCTTGGTATGCATCACAACACCAATCC
CAAAACATCGATCCTACTATCTTTTATGCTGAGATTGCTTCTATTGCCAA
TGAACTGTTGTTGTGTTATTATGAGCTGCAACTTTATAAAAATAACCACA
AGCAGTTAATTGCTAGTTTATTGAGTCAAATCAACCATTTTTTTGGCGCT
ACTACAAGACAAATAATGTTTTCACAATTTGAAAAAGATACGCTTTATTT
AATCAGAGTTAACCAGAAACCTGACTTTAAAACTTTGATTAAAATTTATG
CAAATACTGCGGTTAAATACCAAGGTTTTAAACCTGAAGTAGTTGCTAAT
AAACTAAAAAAGACCCAGTATCAAAAATCATTGTCACACATCATTGCTAT
CCCCCATTTTTATGCAGGTAACTTCTATGTTTACAAGTATGCCATTGGCC
AAGTTGCAGGTATTTTAGTAGCTAAAAAAATTAATAGTGGTGATAAAAAG
ATGAAAGATAATTACTTTAAATTCCTCAGTTCAGGTTCTAGTTTAGCGCC
ACTTGAAACCATTAAACTCTTAGGGATTGACCTTACTTCACCCCAACCCT
GACAAGAAGCACATAACGAAGTAAAGCGTTGGCTTAAAATTGTGAAACAA
AGCTTTAAAAAACTCCAAAAATAA
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1824
View in model
Transcription unit TU_113 View in model
Empirical formula (pH 7.5) H19503C17678N7434O12656P1824
Molecular weight (pH 7.5; Da) 595092.65 View in model
 
Functional genomics
Is essential
No (Show evidence)
View in model
Relative expression
0.560583 (dimensionless) (Show evidence)
View in model
Half life
4.425 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
18802100
pI 3.85
 
Comments
Comments cleaves peptides 5-21 aa long [PUB_0035]; cleaves after hydrophobic and basic residues [PUB_0035]; broad specificity, never cleaves bonds involving acidic residues [PUB_0036]
References
  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Chao SH, Cheng TH, Shaw CY, Lee MH, Hsu YH, Tsai YC. Characterization of a novel PepF-like oligopeptidase secreted by Bacillus amyloliquefaciens 23-7A. Appl Environ Microbiol 72, 968-71 (2006). WholeCell: PUB_0035, PubMed: 16391147

  3. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  4. Monnet V, Nardi M, Chopin A, Chopin MC, Gripon JC. Biochemical and genetic characterization of PepF, an oligopeptidase from Lactococcus lactis. J Biol Chem 269, 32070-6 (1994). WholeCell: PUB_0036, PubMed: 7798200

  5. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:18
Last updated 2012-10-01 15:13:05