MG_140 – putative ATP-dependent helicase

Name
WID MG_140 View in model
Name putative ATP-dependent helicase View in model
Protein product MG_140_MONOMER
Cross references GenBank: AAC71358.1, CMR: MG_140, BioCyc: MG_140, SwissProt: P47386
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 173305181647 MGrrnA23S MG_140 MG_141 MG_142 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 175805 (nt), Length: 3342 (nt), Direction: Forward, G/C content: 33.2%, Sequence:
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ATGAACGACTGACAGTGACTGAAAAACAGACTTGTTAATTCCAAAACCAA
ATCTGTTAGTTTCTGATTACCACAAACCTCAAGTAATATCATTGATATTG
CTGAATTAATTAAGTGCTGTAGTGAGTTAAAAAACACTTCTATTAATGGT
TTAATTGACTTTTTGAACCAACAGGATAAACTTGAATTTAACTTAACAAG
ATTAAAGGAGATAGATGTTGAAGATGGTAAGCAACTATTTGGGATAGAAA
CTAGTGTTTATAAACACTTTCAAAATGAAATTGCTCGTTTTTATAAACAA
GTAAACAAACACTTTCGTGAAACAGGTAGTGAAAGTTTGTTTTTAGCTTT
ACCAGTTATTGAAGGGATTAATGAGTTTAACGATATCTTTCGAGCTCCAT
TACTTTATGTTGGAGTTAAACTCAAAGTTTCCCCACGCTTTGAACGTTTC
TGATTAGAAATTAACAAAGAAGAGATCTTTTTAAACCCTACTATTATTGG
AGTTGAAACCAACAAACGTAATAGTTTGTTTAAAAATAACTATGATACTA
CTAAGATAGATGTTAATGATGCTTTAAAGGTATTTAGTGAACTTGAATAT
GAGTTTAGAATGCCTTTAACTTCTGAATTGAAGAGTTTTAGTAAAAAAGC
AAAGAGTGATTTTAATACTGAAAAACGAACTAACTATCTTATTAACAACG
TTCTTTTGGGGATCTTTGATGTTAAGGGTGATCAGCTGTTCCAAAACTTT
AATGAGATTCTAAACACTGATCCTGATGTATTAGATGAACTTCTAAAAGA
TAGAAGAGATCTGTTGTTAGAAAACCGGGAATTCCGTGAACAATTTGATT
TAAAAGATACCTATCTCTTCAGTCACCTTGATATCTACCAACAGTATGCA
GTTAAGCAAGCTTTACTTGGTGATTTAATTATTGAAGGCCCACCTGGCAC
AGGGAAATCTGAAACAATTGTTAATATCTTAGTTAACCTTGTTTTAAACA
ACAAAAAAGTATTGTTTGTTTCTGAAAAAGTAACTGCACTTGATGTTGTT
TACAACCGTCTTGGTAGTTTTAAACACATCGCACTTTTCAACGCTAGTGT
TGCTGCTGAAAAGAAACGCTTTTATAACCAGTTTGCTGAGTTTGAAACTT
ATTTCACTACTTACTTTTCCAAGAAAGATTTGGATGCTACTTTACCAACA
TTTGAAGGTAAATGGGTGGATGATATTTTAGGGGCATTTCAAGCATTACA
AGCTCTTTATGACACCAAGATAAATTCTGGTGAAAATCTGTTTAGTTTCA
AAGAGATTGTCAGTAGCTTTCAGATGTTGGATGCTAGTTACATCAAGATT
AAAGAATATGAACGTTTTGATGAGTGAGTGCGCGTCTTTTCAAATCCATT
GTGATTAGAAAAACACTTAAGTTACCAAGAGTTGAAAAAAGAACTTAGTC
AGCGCTGGAATGGTATTGATAATTTCTATCAGTTGCAATCGCTTCTAAAC
CAAACCAAAAAACGAAAGGTCTTAAACTATGTGTTGGAACACTTTGAACA
GTTTAATACAGTTATCAGTCCTAAGCATGTTTTGTTCTACAAGCCTAGCA
ATAAATCACAATTGCTCTTAAAACAACTGAAACAGGATGTTGAACAATAC
ACTAGTTTACAACGTTTCCAATCTCCTACTAAGTTTGAAACAATCAAGTT
GAATTTCATCAACCAAGTTAATGAAAACCCAACCCCATGGTTCTTTTCTT
GATTTATCCAATTTCATGCCAAGCCACTGTTGGAAAAACTCGTTAGTTTT
GAGTCAAACATTATTAAAACAAAACAAGCTTATCTTAATGGGATTGAAAG
CTATGTAGCAAGTTGTAAGAAACTGCTTAAAACAACTATTTTAAACAACT
TTTTTCAGCTTTATCAAACTAATAAAGATGAACTATTGGAGATCTGCAGA
CAAGCAAAAAACCCAGTTTTAAAAGAGATTACTTGGTGGTTTAAAAAACA
TTTTGAACTCTTAAAAAAACTCTTTCCAGTGCACATTATGACCCTTGAGT
CTGCAGCAACTCTAACCCCTAACCAACGTGGTTTGTATGACTATGTGGTT
ATTGATGAAGCTAGTCAAGTATTTCTAGAAAGAGCAATTCCTATCTTATT
TAGAGCTGATAAGTACATTATTGCAGGGGACACTAAACAGTTAAAACCAG
CTAACTTCTTCCAATCACGTGCTGAGTATGATGTTGATGAGGAGTTTGAA
GATGGCAATATAGAAGCTGCTGTTCACTCCAGTTCTCTACTTCATTTTTT
AAAGAACCGTTCAAGAATCTTAACTTTACTTAAGTTCCACTACCGCAGTG
ATTCAGCTGATCTGATTGCTTTTACTAATAACAGGATCTATGACAATGAA
TTAATCTTTATGAATAAAGCTAATGCTGATCAAAGGGTTTTTATTGTCCA
TGATGTAATAGATGGTATCTGAAAAAACAACCGGAATCTCCAAGAAGCAC
GTGATGTTGTGCAACGCTTAGAACAACTAACAACAACCAATGATTACAAA
AAGTCACTTGGTGTAATTTGTTTCAATAAAAACCAAGCAGATCTAATTGA
ATATCTAATTGATAAACAAAACAATCCATTACTTAACGAGTGAAGAGAAA
GACAAAATGATGTTGGTGAATATGAAGGATTATTTGTGAAAAACATTGAA
AATGTGCAGGGTGATGAAAGGGATATCATCATCTTTTCTTTAGGTTATGA
TCGTTCAGTAAATAGTTATGGTCCTATTAGTAAGCAAGGTGGAGAAAACA
GACTAAATGTAGCGATAACTAGAGCTAAACAAAGAATAGAACTGTTTAAG
ACAAATAGAGGAGAAGACTACAATGGTTTAAGCTCCAGTTCCTTGGGTAG
TAAACTGTTAGTTGAATATCTGCTTTATTGTGAAGCGATGGCTAAAAACC
AGGGTGAGAAAATCACTTTTCAAGCGGTAAAAAGAAAAGAAACAAAAGCA
AAGTATGAACTGGCAGTTGAAAATGATTTCTTCAACCAACTGCAGGCAAT
TTTTGGTGGAGAGTTTGAGATTAAACGTAACGTTAATGAAGGGGCTTACT
TTTTCTCATTTGTCTTTTACTTTAATAATATCCCTTATCTTGCCATTGAC
TTTAACATCCCCATTCCCACTTCAAGAAAACAAGTTATGGAAGGGATTTT
ATACCGTGAACAGTTTCTCAAAAAACGTCAATGGAACCTAATTAACATCT
GGATTGATGAGTGAAAATTAAACCCAATTGGGGTGATTTCTAAAATCAGA
TCAAGTTTAGCAGTGCATCAAAACCAGCATGAAGAAATATAA
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3342
View in model
Transcription unit TU_089 View in model
Empirical formula (pH 7.5) H35713C32370N13560O23262P3342
Molecular weight (pH 7.5; Da) 1090404.83 View in model
 
Functional genomics
Is essential
No (Show evidence)
View in model
Relative expression
1.29325 (dimensionless) (Show evidence)
View in model
Half life
4.425 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
34521500
pI 3.94
 
Comments
Comments DNA2/NAM7 helicase family [PUB_0096]; DNA2 protein is a eukaryote-specific helicase–nuclease fusion that has been horizontally acquired by cyanobacteria, probably from the plant lineage [PUB_0198]; implicated in Okazaki fragment processing [PUB_0199];
References
  1. Aravind L, Makarova KS, Koonin EV. SURVEY AND SUMMARY: holliday junction resolvases and related nucleases: identification of new families, phyletic distribution and evolutionary trajectories. Nucleic Acids Res 28, 3417-32 (2000). WholeCell: PUB_0198, PubMed: 10982859

  2. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  3. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  4. Mimitou EP, Symington LS. Nucleases and helicases take center stage in homologous recombination. Trends Biochem Sci 34, 264-72 (2009). WholeCell: PUB_0199, PubMed: 19375328

  5. UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/

  6. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:16
Last updated 2012-10-01 15:12:58