MG_138 – Translation elongation factor 4

Name
WID MG_138 View in model
Name Translation elongation factor 4 View in model
Symbol lepA
Synonyms EF4
Protein product MG_138_MONOMER
Cross references GenBank: AAC71355.1, CMR: MG_138, BioCyc: MG_138, SwissProt: P47384
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 161091167888 MG_136 MG_137 MG_138 MG_139 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 163591 (nt), Length: 1797 (nt), Direction: Forward, G/C content: 33.4%, Sequence:
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ATGGAACAAAAAAACATTAGAAATTTTTCTATTATTGCCCATATTGATCA
TGGTAAATCTACCTTATCAGACCGCTTGTTAGAACATAGTTTAGGCTTTG
AAAAAAGACTATTACAAGCGCAAATGCTTGATACTATGGAGATTGAAAGA
GAAAGGGGTATTACCATTAAATTAAATGCTGTTGAATTGAAAATTAATGT
TGATAACAACAACTATCTTTTTCATTTAATTGACACCCCTGGGCATGTTG
ATTTTACTTATGAAGTGTCTCGTTCTTTAGCAGCTTGTGAGGGAGTTTTA
TTGTTAGTAGATGCAACCCAAGGAATTCAAGCACAAACGATTTCCAATGC
TTATCTTGCGTTGGAAAATAACCTGGAAATTATCCCAGTTATTAACAAGA
TAGATATGGATAATGCTGATATTGAAACAACAAAAGATTCACTCCATAAC
TTATTAGGAGTTGAAAAGAACAGTATCTGTTTAGTATCTGCAAAAGCTAA
CTTAGGGATTGATCAGTTAATTCAAACAATTATAGCTAAGATCCCCCCAC
CAAAAGGAGAAATTAATAGACCTTTAAAAGCATTACTCTTTGATAGTTAC
TATGATCCTTACAAGGGGGTTGTTTGTTTTATTAGGGTATTTGATGGTTG
TTTAAAGGTTAATGATAAGGTTCGTTTTATTAAAAGTAATTCTGTTTACC
AAATTGTGGAACTAGGGGTTAAAACCCCATTTTTTGAAAAAAGAGATCAA
TTGCAAGCAGGAGATGTTGGTTGGTTTTCAGCAGGGATAAAAAAACTTCG
TGATGTTGGGGTTGGTGATACTATTGTTAGTTTTGATGATCAATTTACAA
AACCCCTAGCAGGTTATAAAAAGATCTTACCCATGATCTATTGTGGTTTA
TATCCAGTTGATAACAGTGATTATCAAAACCTCAAGTTAGCGATGGAAAA
GATCATAATCAGTGATGCAGCATTGGAATATGAATATGAAACATCCCAAG
CGTTAGGTTTTGGGGTTAGGTGTGGTTTTCTAGGTCTTTTACATATGGAT
GTTATTAAAGAAAGATTGGAAAGAGAATACAACCTAAAACTCATCTCAGC
TCCCCCTTCAGTTGTATATAAGGTGTTGTTAACAAATGGTAAAGAGATTA
GTATTGACAATCCCTCTTTGTTACCAGAACGCTCCAAGATTAAAGCAATC
AGTGAACCATTTGTAAAAGTCTTTATTGATTTACCTGATCAATATTTGGG
CAGTGTTATTGATTTATGCCAAAACTTCAGGGGTCAATATGAAAGTTTAA
ATGAGATTGATATCAACAGAAAAAGAATCTGTTATCTGATGCCTTTAGGG
GAAATTATCTACAGTTTTTTTGATAAGTTAAAGTCGATTAGTAAGGGTTA
TGCATCGTTAAACTATGAGTTTTATAACTACCAACATAGTCAACTGGAAA
AAGTTGAGATCATGTTAAACAAACAAAAGATTGATGCATTATCTTTTATC
AGTCATAAAGACTTTGCTTTTAAGCGGGCAAAAAAGTTTTGCACTAAGCT
CAAAGAATTGATTCCCAAGCATCTGTTTGAGATCCCTATCCAAGCAACAA
TAGGGAGTAAAGTAATAGCAAGAGAGACAATCAAAGCAGTTAGAAAGGAT
GTAATAGCTAAACTTTATGGAGGGGATGTTAGTAGAAAAAAGAAGTTATT
AGAGAAGCAAAAAGAGGGTAAAAAACGCTTGAAAGCAGTTGGGAGTGTTC
AATTACCCCAAGAGCTATTTAGTCATTTGCTGAAAGATGAAGATTAA
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1797
View in model
Transcription unit TU_086 View in model
Empirical formula (pH 7.5) H19203C17405N7290O12514P1797
Molecular weight (pH 7.5; Da) 586386.19 View in model
 
Functional genomics
Is essential
No (Show evidence)
View in model
Relative expression
0.560583 (dimensionless) (Show evidence)
View in model
Half life
2.4 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
18703800
pI 3.98
 
Comments
Comments GTP-binding elongation factor family [PUB_0096]; significant homology to EF-G, EF-Tu [PUB_0110]; back-translocates posttranslocational ribosomes, and the results suggest that it recognizes ribosomes after a defective translocation reaction and induces a back-translocation, thus giving EF-G a second chance to translocate the tRNAs correctly [PUB_0219]; rate of defective EF-G incorporation and translation errors increase under high ionic strength (eg. high Mg2+) [PUB_0219]; lepA improves fidelity of translation [PUB_0219];
References
  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/

  3. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  4. Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN, Nierhaus KH. The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome. Cell 127, 721-33 (2006). WholeCell: PUB_0219, PubMed: 17110332

  5. UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/

  6. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:16
Last updated 2012-10-01 15:12:58