MG_127 – Spx subfamily protein

Name
WID	MG_127
Name	Spx subfamily protein
Symbol	spx
Synonyms	yjbD
Protein product	MG_127_MONOMER
Cross references	GenBank: AAC71345.1, CMR: MG_127, BioCyc: MG_127, SwissProt: P47373
Homologs	B. subtilis: BSU11500 M. pneumoniae: MPN_266 S. oneidensis: SO_2470

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 154999 (nt), Length: 438 (nt), Direction: Forward, G/C content: 31.5%, Sequence: 1 51 101 151 201 251 301 351 401 ATGAAAAAAGCGGGTAAAAAAAATAGTGATGCAGGTAAGACTTTTATCTT AATCTCATCATCCTGTTCATCCTGTCAGAAAGCCATTGAGTTTTTTGATC AAAACAAAATTAGCTATGTTGTTGAGAATTTTTATAAAAAACCAATTAGT GATAAGCGTTTTAAAGATATTTTAAGTCTTAGTGAGGATGGTACTGAAAG TTTGTTTTCCAAACGTGCTGATCAGATTAAAGCAACTAACAGTGTTAGTG TTGAAGAGTTGAGTATCAGTGAATTAATTAAGCTAGTTAGAGAACGTCCT TCTTTACTAAGAAGGCCTATTATCATCCAATATAATTCTTCAGGAATTCC TAAAAGGATGCGAATTGGTTATAACTCATCTGAAATTAAGGTCTTTGAGC GTAAACTAATAGAACCAAAGCCTATAATACAACAATAA 50 100 150 200 250 300 350 400 438
Transcription unit	TU_078
Empirical formula (pH 7.5)	H₄₆₇₉C₄₂₄₀N₁₇₇₀O₃₀₄₇P₄₃₈
Molecular weight (pH 7.5; Da)	142749.89

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.96428 (dimensionless) (Show evidence) Value: 1.96428 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	4554200
pI	3.95

Comments
Comments	major function is to restore thiol balance under disulfide stress through transcriptional regulation [PUB_0112]; upregulatex trxA, trxB [PUB_0112]; Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress [PUB_0096]; inhibits transcription by binding to the C-terminal domain of the alpha subunit of RNAP, disrupting complex formation between RNAP and certain transcriptional activator proteins like ResD and ComA. In response to oxidative stress, Spx can also activate transcription, making it a general regulator that exerts both positive and negative control over transcription initiation [PUB_0110]; regulated by clpP protease [PUB_0111];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Nakano S, Zheng G, Nakano MM, Zuber P. Multiple pathways of Spx (YjbD) proteolysis in Bacillus subtilis. J Bacteriol 184, 3664-70 (2002). WholeCell: PUB_0111, PubMed: 12057962 Newberry KJ, Nakano S, Zuber P, Brennan RG. Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase. Proc Natl Acad Sci U S A 102, 15839-44 (2005). WholeCell: PUB_0112, PubMed: 16249335 ... 2 more UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/ Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:15
Last updated	2012-10-01 15:12:57


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