MG_094_HEXAMER – replicative DNA helicase

Name
WID MG_094_HEXAMER View in model
Name replicative DNA helicase View in model
Cross references PDB: 1JWE
 
Structure
Biosynthesis [c]: (6) MG_094_MONOMERMG_094_HEXAMER View in model
No. subunits 6
DNA footprint Length: 20 (nt), Binding: ssDNA, Region: xsDNA View in model
Empirical formula (pH 7.5) H23028C14454N3942O4362S48
Molecular weight (pH 7.5; Da) 323355.49 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
-140280.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
 
Synthesis
Formation process Process_MacromolecularComplexation View in model
Localization c
 
Function
Enzyme DNA unwinding
[c]: ATP + H2OADP + H + PI
View in model
Reaction participant View in model
Complex subunit View in model
 
Parameters
Parameters Parameter_0145: rnaPolymeraseCollisionMeanDwellTime = 1.7 s View in model
 
Comments
Comments Composition [PUB_0201]. DNA-binding DnaB occipies 17-23 nt [PUB_0628].
References
  1. Bujalowski W, Jezewska MJ. Interactions of Escherichia coli primary replicative helicase DnaB protein with single-stranded DNA. The nucleic acid does not wrap around the protein hexamer. Biochemistry 34, 8513-9 (1995). WholeCell: PUB_0628, PubMed: 7612593

  2. Mitkova AV, Khopde SM, Biswas SB. Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis. J Biol Chem 278, 52253-61 (2003). WholeCell: PUB_0201, PubMed: 14557266

 
Metadata
Created 2012-10-01 15:07:37
Last updated 2012-10-01 15:14:05