MG_091_TETRAMER – single-strand binding protein family, tetramer

Name
WID MG_091_TETRAMER View in model
Name single-strand binding protein family, tetramer View in model
 
Structure
Biosynthesis [c]: (4) MG_091_MONOMERMG_091_TETRAMER View in model
No. subunits 4
DNA footprint Length: 72 (nt), Binding: ssDNA, Region: ssDNA View in model
Empirical formula (pH 7.5) H4848C3196N828O1024S20
Molecular weight (pH 7.5; Da) 71894.73 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
-34420.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
 
Synthesis
Formation process Process_MacromolecularComplexation View in model
Localization c
 
Function
Complex subunit View in model
 
Comments
Comments Composition [PUB_0334]. DNA-binding 8 SSB monomers bind per 145 nt [PUB_0637]. Clusters of 8 SSB monomers, or beads, are spaced by 30 nt [PUB_0637]. Thus the spacing between SSB beads is 1 per 175 nt [PUB_0637]. Footprint of a single SSB is 5 nt [PUB_0734]. This value must be larger than dnaPolymeraseElongationRate.
References
  1. Bujalowski W, Lohman TM. Monomers of the Escherichia coli SSB-1 mutant protein bind single-stranded DNA. J Mol Biol 217, 63-74 (1991). WholeCell: PUB_0334, PubMed: 1988680

  2. Chrysogelos S, Griffith J. Escherichia coli single-strand binding protein organizes single-stranded DNA in nucleosome-like units. Proc Natl Acad Sci U S A 79, 5803-7 (1982). WholeCell: PUB_0637, PubMed: 6764531

  3. Wadsworth RI, White MF. Identification and properties of the crenarchaeal single-stranded DNA binding protein from Sulfolobus solfataricus. Nucleic Acids Res 29, 914-20 (2001). WholeCell: PUB_0734, PubMed: 11160923

 
Metadata
Created 2012-10-01 15:07:37
Last updated 2012-10-01 15:14:05