MG_091_OCTAMER – single-strand binding protein family, octamer

Name
WID MG_091_OCTAMER View in model
Name single-strand binding protein family, octamer View in model
 
Structure
Biosynthesis [c]: (2) MG_091_TETRAMERMG_091_OCTAMER View in model
No. subunits 0
DNA footprint Length: 145 (nt), Binding: ssDNA, Region: ssDNA View in model
Empirical formula (pH 7.5) H9696C6392N1656O2048S40
Molecular weight (pH 7.5; Da) 143789.46 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
68840.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
 
Synthesis
Formation process Process_Replication View in model
Localization c
 
Function
Reaction participant View in model
Complex subunit single-strand binding protein family, octamer
[c]: (2) MG_091_TETRAMERMG_091_OCTAMER
View in model
 
Parameters
Parameters View in model
 
Comments
Comments DNA-binding 8 SSB monomers bind per 145 nt [PUB_0637]. Clusters of 8 SSB monomers, or beads, are spaced by 30 nt [PUB_0637]. Thus the spacing between SSB beads is 1 per 175 nt [PUB_0637]. Footprint of a single SSB is 5 nt [PUB_0734].
References
  1. Chrysogelos S, Griffith J. Escherichia coli single-strand binding protein organizes single-stranded DNA in nucleosome-like units. Proc Natl Acad Sci U S A 79, 5803-7 (1982). WholeCell: PUB_0637, PubMed: 6764531

  2. Wadsworth RI, White MF. Identification and properties of the crenarchaeal single-stranded DNA binding protein from Sulfolobus solfataricus. Nucleic Acids Res 29, 914-20 (2001). WholeCell: PUB_0734, PubMed: 11160923

 
Metadata
Created 2012-10-01 15:07:37
Last updated 2012-10-01 15:14:05