MG_072 – preprotein translocase, SecA subunit

Name
WID MG_072 View in model
Name preprotein translocase, SecA subunit View in model
Symbol secA
Protein product MG_072_MONOMER
Cross references GenBank: AAC71290.1, CMR: MG_072, BioCyc: MG_072, SwissProt: P47318
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 9203599456 MG_071 MG_072 MG_073 MG_074 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 94535 (nt), Length: 2421 (nt), Direction: Forward, G/C content: 35.8%, Sequence:
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2401
ATGGCAATATTTAACTTCCTTAAGTTAATTTCACCCAAAAACAGAATTCT
CAGTAAGGCAAATAGGATTGCCAGTGAGGTTGAGAGTTATAAAAACTACT
ACCGTAACTTAACTGATCAACAGTTATTTGAAGAGTCAAATAAACTAGTT
GATCTTGTCACTAAGCAAAATTACACCATTCTAGATGTTTGTGTTGCTGC
ACTTGCTTTAATTAGAGAAGTGGTTTACCGTGAGACTGGTGAATTTGCAT
ATAGGGTGCAGATCATAGGAGCTTTTATTGTTTTAAGTGGTGATTTTGCT
GAGATGATGACTGGTGAAGGTAAGACCTTAACCATTGTTTTAGCAGCATA
CGTTTCTGCACTTGAAAAGCGTGGTGTGCATGTTGTTACTGTTAATGAAT
ATCTAGCTCAAAGGGATGCTAATAATGCAATGAAGATCTTAAAACGGGTT
GGGATGAGTGTCGGTTGTAACTTTGCTAATCTCTCCCCTCAGCTAAAACA
AGCTGCATTTAATTGCGATGTTACCTACACCACTAACAGTGAACTGGGGT
TTGATTATCTTAGAGATAACATGGTCCACAGTTATCAAGATAAGAAGATC
AGAGAGTTGCACTTTGCAATAGTTGATGAAGGTGATTCAGTTTTAATTGA
TGAGGCGCGAACGCCTTTAATTATTTCAGGTCCTAGTAAAAATGAGTTTG
GGTTATATGTTGCAGTTGATCGATTTGTTAAATCATTAACTGAACAGGAG
TTTAAGATTGACCCTGAATCACGTGCTGCTTCTTTAACTGAACTTGGGAT
TAAAAAAGCAGAGCAAACATTTAAAAAAGAAAACCTTTTTGCTTTGGAAA
ACAGTGATCTTTTTCACAAGATCATGAATGGTTTGACTGCTGTGAAAGTT
TTTGAACAGGGCAAAGAGTACATTGTTCGTGATGGCAAGGTTTTAATTGT
TGATCACTTTACAGGTAGGATATTGGAAGGGAGAAGTTACAGTAATGGCT
TACAACAAGCTGTACAAGCCAAAGAATATGTTGAGATAGAACCTGAAAAT
GTGATAGTAGCTACCATTACCTACCAATCCTTCTTTAGGCTATACAACCG
CTTAGCAGCAGTATCAGGTACTGCTTTAACTGAATCAGAGGAGTTTCTCA
AGATTTATAACATGGTTGTAGTACCAGTGCCAACTAACCGTCCTAACATC
AGAAAAGACCGTTCTGATAGTGTATTTGGTACCCCACAAATTAAGTGAAT
GGCAGTTGTTAAAGAGATAAAAAAGATCCATGAAACTTCTCGACCTATTC
TGATTGGAACTGCTAACATAGATGATTCTGAACTCTTACATAATCTGTTA
CTAGAAGCTAATATTCCCCATGAGGTTTTAAATGCTAAAAACCATTCAAG
AGAAGCGGAGATAGTAACTAAAGCAGGACAGAAGAATGCAGTTACTATTT
CAACTAACATGGCTGGAAGAGGAACTGATATCCGTTTAGGTGAAGGGGTT
GCTGAAATGGGTGGTCTTTATGTATTGGGAACTGAAAGAAATGAGTCAAG
AAGGATTGATAACCAACTAAGAGGGAGAGCTGCTAGACAAGGTGATAAAG
GGGAAACTAAGTTCTTTATCTCACTAGGTGATTCATTGTTTAAACGTTTT
GCTCATGACAAGATTGAAAGAGCGATTAGCAAATTAGGTAATGAAACATT
TGACAGTGCCTTCTTTTCCAAAATGTTAAGTAGAACCCAAAAACGGGTGG
AAGCAATTAACTTTGACACTAGAAAAAACCTGATTGATTATGACCATGTT
CTTGCAAGTCAAAGGGAATTGATTTACAAACAACGTGATAAGTTTTTATT
AGCAAACGATTTAAGTGAAATGATCGACAAAATGCTAGAAAAGTTTGTAC
AACAGTTTTGTGATCAATATAGAAACCAAAAGAACCAAAACTTAATTAAT
CACATTGCACTAGCAGAAGCTTTAAATCTTGAGATGAACATGCAAAACAC
CATTAATCCAAAGGTGTTTGAAAACATGACTTTTGATGTTGCTGTTGATA
AAACCCGTAACTTAGTAGCTAAAAAGATTAGTGATAAAGTTAATGTTTTG
ACCAAACCAATTGCTTTAAACAGGTTTCGTGACATTATCATAACTTCGAT
GGATAAACATTGAACTGAACACTTGGATAGTGTTTTTAAGTTAAGAGAAG
GGGTTGTACTTCGTTCTATGGAACATACGAGTCCTTTAAATGTTTACATT
AAAGAAACAGATATCCTTTTTAAAACAATGTTGCAAAAGATTGCTCAAGA
TGTCATTGTGCAAATTGCTAACCTCACAACTCCAGATGAATTTGATCATA
GCTTAATGCAAGCCAATGCTTTAAAGAAACTAGCAGCAATTAAAGCAGAT
GAAAAATCAAACCAAGAGTAA
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2421
View in model
Transcription unit TU_046 View in model
Empirical formula (pH 7.5) H25911C23489N9942O16835P2421
Molecular weight (pH 7.5; Da) 791827.11 View in model
 
Functional genomics
Is essential
Yes (Show evidence)
View in model
Relative expression
0.560583 (dimensionless) (Show evidence)
View in model
Half life
2.6 (min) (Show evidence)
View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
24993900
pI 3.90
 
Comments
Comments Translocates unfolding proteins into and through the cell membrane. Tomkiewicz et al observed a kinetic rate of 270 pmol aa/min for the E. coli SecA translocase [PUB_0001]. Tomkiewicz et al and Doyle et al observed an energetic cost of 20-50 aa/ATP [PUB_0002, PUB_0003]. Dalbey and von Heijne observed an energetic cost of 4-5 DA/ATP [PUB_0003].
References
  1. Eds Dalbey RE, von Heijne G. Protein Targeting, Transport, and Translocation. Academic Press, San Diego (2002). WholeCell: PUB_0003, ISBN: 9780122007316

  2. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  3. Doyle SM, Bilsel O, Teschke CM. SecA folding kinetics: a large dimeric protein rapidly forms multiple native states. J Mol Biol 341, 199-214 (2004). WholeCell: PUB_0002, PubMed: 15312773

  4. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  5. Tomkiewicz D, Nouwen N, van Leeuwen R, Tans S, Driessen AJ. SecA supports a constant rate of preprotein translocation. J Biol Chem 281, 15709-13 (2006). WholeCell: PUB_0001, PubMed: 16601117

  6. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

 
Metadata
Created 2012-10-01 15:07:13
Last updated 2012-10-01 15:12:48