MG_057 – small primase-like protein

Name
WID	MG_057
Name	small primase-like protein
Protein product	MG_057_MONOMER
Cross references	GenBank: AAC71274.1, CMR: MG_057, BioCyc: MG_057, SwissProt: P47303
Homologs	B. subtilis: BSU00410 M. pneumoniae: MPN_072

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 65712 (nt), Length: 537 (nt), Direction: Reverse, G/C content: 27.4%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 ATGGATCAAAAAGCACGTATAAAAATTGATGGGGTAATTGTTTGTGAGGG TAAAACTGACCAAGCTAAATTACAGCAAATTTTTGATGTAGATGTAATTA CAACAAATGGTTCAGCTTTAAAAAAAGAAACTATTAACCTAATTAAAAAA ATCAGTGAAAAGCAAACTGTTATCTTGTTATTAGATCCAGATCAACAAGG CAAAAAGATTAGAAGTAAATTAGAACAACATCTAACAAACTATTACAACT GTTATGTTGATATGAATGCAAGGTTAAAAAACGCTAAAAAAGCTGGTATT GCTGAAATGGAAACTAGTGCTTTAATTGCTGCTTTAAATAATCGTGTTGC AATCACAAAAAATGCTAATCAAAGCATATTATGAGATCAGTATTTAAGCT TAAAGTTAAATGATAAGAAAAAGCGATTACTTTTATGCAATAATCTTAAT TTACCTTATTTTAACCACAAGCAACTATTTAAAAAACTTAACTTATTAAA TCTAACTTTTCAAGATGTATGAAAACACTTAAAGTAG 50 100 150 200 250 300 350 400 450 500 537
Transcription unit	TU_032
Empirical formula (pH 7.5)	H₅₇₄₇C₅₂₀₉N₂₂₀₂O₃₆₉₂P₅₃₇
Molecular weight (pH 7.5; Da)	174901.61

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	5658600
pI	3.73

Comments
Comments	Contain toprim domain found in bacterial primases [PUB_0202]. Glass manual annotates this gene as ribonuclease MS [PUB_0764].
References	Aravind L, Leipe DD, Koonin EV. Toprim--a conserved catalytic domain in type IA and II topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins. Nucleic Acids Res 26, 4205-13 (1998). WholeCell: PUB_0202, PubMed: 9722641 Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Goetz P. Cell-cycle-regulated expression and subcellular localization of the Caulobacter crescentus SMC chromosome structural protein. (2011). WholeCell: PUB_0764, None Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:12
Last updated	2012-10-01 15:12:46


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