FolD2 – methenyltetrahydrofolate cyclohydrolase

Name
WID FolD2 View in model
Name methenyltetrahydrofolate cyclohydrolase View in model
Cross references SABIO-RK: 20749, EC: 3.5.4.9, BioCyc: METHENYLTHFCYCLOHYDRO-RXN, BiGG: MTHFC, KEGG: R01655
 
Classification
Type chemical View in model
 
Reaction
Stoichiometry [c]: H2O + METHFFTHF10 + H View in model
 
Catalysis
Enzyme MG_013_DIMER [c] View in model
Optimal pH
7.4 (dimensionless)
Optimal temperature
27.0 (C)
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
ΔG (pH 7.5, 25C, I = 0; kJ mol‑1) -0.500
Keq
2.29442124839 ()
 
Kinetics
Forward kinetics
v = Vmax * [METHF] / (Km + [METHF]); Km = 74 (nM), Vmax = 31.0 U/mg (Show evidence)
View in model
 
Associations
Pathways FOLATE_METABOLISM
Process Process_Metabolism View in model
 
Comments
Comments Vmax=31 U/mg was observed in E. coli [PUB_0570]. KM=74 μM and Vmax=0.088 U/mg were observed in Oryctolagus [PUB_0100].
References
  1. Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617

  2. Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

 
Metadata
Created 2012-10-01 15:08:34
Last updated 2012-10-01 15:16:29