DNA_POLYMERASE_GAMMA_COMPLEX – DNA-directed DNA polymerase gamma complex

Name
WID DNA_POLYMERASE_GAMMA_COMPLEX View in model
Name DNA-directed DNA polymerase gamma complex View in model
 
Structure
Biosynthesis [c]: MG_007_MONOMER + MG_315_MONOMER + (4) MG_419_MONOMERDNA_POLYMERASE_GAMMA_COMPLEX View in model
No. subunits 6
DNA footprint Length: 26 (nt), Binding: ssDNA, Region: xsDNA View in model
Empirical formula (pH 7.5) H24404C15451N3970O4508S57
Molecular weight (pH 7.5; Da) 339733.72 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
-244200.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
 
Synthesis
Formation process Process_MacromolecularComplexation View in model
Localization c
 
Function
Complex subunit View in model
 
Comments
Comments Composition [PUB_0312, PUB_0313, PUB_0314]. DNA-binding DNA polymerase III preinitiation complex (γ-complex and β-subunit) has 51 nt footprint [PUB_0743]. Assuming the γ-complex and β-subunit contribute equally to the footprint, we conclude that each has a footprint 25-26 nt.
References
  1. Bruck I, O'Donnell M. The DNA replication machine of a gram-positive organism. J Biol Chem 275, 28971-83 (2000). WholeCell: PUB_0314, PubMed: 10878011

  2. Kelman Z, O'Donnell M. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu Rev Biochem 64, 171-200 (1995). WholeCell: PUB_0312, PubMed: 7574479

  3. Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev 62, 1094-156 (1998). WholeCell: PUB_0313, PubMed: 9841667

  4. Reems JA, McHenry CS. Escherichia coli DNA polymerase III holoenzyme footprints three helical turns of its primer. J Biol Chem 269, 33091-6 (1994). WholeCell: PUB_0743, PubMed: 7806536

 
Metadata
Created 2012-10-01 15:07:36
Last updated 2012-10-01 15:14:01