DNA_GYRASE – DNA gyrase

Name
WID DNA_GYRASE View in model
Name DNA gyrase View in model
Cross references PDB: 1KZN
 
Structure
Biosynthesis [c]: (2) MG_003_MONOMER + (2) MG_004_MONOMERDNA_GYRASE View in model
No. subunits 4
DNA footprint Length: 140 (nt), Binding: dsDNA, Region: dsDNA View in model
Empirical formula (pH 7.5) H23816C14868N4088O4516S78
Molecular weight (pH 7.5; Da) 334592.98 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
-179430.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
 
Synthesis
Formation process Process_MacromolecularComplexation View in model
Localization c
 
Regulation
Regulatory rule !ciprofloxacin & !difloxacin & !sparfloxacin View in model
 
Function
Enzyme Negative supercoiling (gyrase)
[c]: (2) ATP + (2) H2O ⇒ (2) ADP + (2) H + (2) PI
View in model
Reaction participant View in model
Complex subunit DNA gyrase
[c]: (2) MG_003_MONOMER + (2) MG_004_MONOMERDNA_GYRASE
View in model
 
Parameters
Parameters View in model
 
Comments
Comments Composition [PUB_0096] DNA footprint Gyrase has 128 [PUB_0693], 140 nt footprint [PUB_0695]. Regulation: Fluoroquinolone antibiotics inhibit topoisomerase II / DNA gyrase [PUB_0847].
References
  1. Knox C, Law V, Jewison T, Liu P, Ly S, Frolkis A, Pon A, Banco K, Mak C, Neveu V, Djoumbou Y, Eisner R, Guo AC, Wishart DS. DrugBank 3.0: a comprehensive resource for 'omics' research on drugs. Nucleic Acids Res 39, D1035-41 (2011). WholeCell: PUB_0847, PubMed: 21059682

  2. Nöllmann M, Stone MD, Bryant Z, Gore J, Crisona NJ, Hong SC, Mitelheiser S, Maxwell A, Bustamante C, Cozzarelli NR. Multiple modes of Escherichia coli DNA gyrase activity revealed by force and torque. Nat Struct Mol Biol 14, 264-71 (2007). WholeCell: PUB_0695, PubMed: 17334374

  3. UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/

  4. Wang JC. DNA topoisomerases. Annu Rev Biochem 65, 635-92 (1996). WholeCell: PUB_0693, PubMed: 8811192

 
Metadata
Created 2012-10-01 15:07:35
Last updated 2012-10-01 15:14:00