DNAProcessiveCleavage_dTMP – DNA cleavage (dTMP)

Name
WID DNAProcessiveCleavage_dTMP View in model
Name DNA cleavage (dTMP) View in model
 
Classification
Type DNA_cleavage View in model
 
Reaction
Stoichiometry [c]: dTpdTp + H2O ⇒ (2) DTMP + H View in model
 
Catalysis
Enzyme MG_190_MONOMER [c] View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
 
Kinetics
Forward kinetics Vmax = 4.44 1/min View in model
 
Associations
Process Process_DNARepair View in model
 
Comments
Comments Yamagata et al measured kcat=0.002 1/s and Km=4.1 μM at 37C [PUB_0552]. Sharma and Rao measured 3.7 nM/s activity for 50 nM RecJ which equates to kcat=4.44 1/min[PUB_0768].
References
  1. Sharma R, Rao DN. Orchestration of Haemophilus influenzae RecJ exonuclease by interaction with single-stranded DNA-binding protein. J Mol Biol 385, 1375-96 (2009). WholeCell: PUB_0768, PubMed: 19094995

  2. Yamagata A, Masui R, Kakuta Y, Kuramitsu S, Fukuyama K. Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain. Nucleic Acids Res 29, 4617-24 (2001). WholeCell: PUB_0552, PubMed: 11713311

 
Metadata
Created 2012-10-01 15:08:33
Last updated 2012-10-01 15:16:28