DNADamage_THY_hm5URA_hydroxyl_radical – DNA radiation (gamma-ray) induced base oxidation (thymine ==> 5-hydroxymethyluracil; hydroxyl radical)

Name
WID DNADamage_THY_hm5URA_hydroxyl_radical View in model
Name DNA radiation (gamma-ray) induced base oxidation (thymine ==> 5-hydroxymethyluracil; hydroxyl radical) View in model
 
Classification
Type radiation_induced_base_oxidation View in model
 
Reaction
Stoichiometry gamma_radiation[e] + hydroxyl_radical[c] + THY[c] ⇒ H[c] + hm5URA[c] View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) True View in model
 
Associations
Process Process_DNADamage View in model
 
Comments
Comments Formed by UV-A photooxidation and induced by ionization radiation through thymidine radicals interacting with molecular oxygen [PUB_0471, PUB_0474]. Reaction rate computed from 1) total gamma ray base damage yield 1.7e-9 1/Gy/DA [PUB_0497], 2) 305 nt/DA, and 3) relative yields of various damaged bases [PUB_0461, PUB_0501].
References
  1. Bjelland S, Eide L, Time RW, Stote R, Eftedal I, Volden G, Seeberg E. Oxidation of thymine to 5-formyluracil in DNA: mechanisms of formation, structural implications, and base excision by human cell free extracts. Biochemistry 34, 14758-64 (1995). WholeCell: PUB_0471, PubMed: 7578084

  2. Dizdaroglu M. Measurement of radiation-induced damage to DNA at the molecular level. Int J Radiat Biol 61, 175-83 (1992). WholeCell: PUB_0501, PubMed: 1351904

  3. Friedberg EC, Walker GC, Siede W, Wood RD, Schultz RA, Ellenberger T. DNA repair and mutagenesis. ASM Press (2006). WholeCell: PUB_0461, ISBN: 9781555813192

  4. Gulston M, Fulford J, Jenner T, de Lara C, O'Neill P. Clustered DNA damage induced by gamma radiation in human fibroblasts (HF19), hamster (V79-4) cells and plasmid DNA is revealed as Fpg and Nth sensitive sites. Nucleic Acids Res 30, 3464-72 (2002). WholeCell: PUB_0497, PubMed: 12140332

  5. Teebor GW, Boorstein RJ, Cadet J. The repairability of oxidative free radical mediated damage to DNA: a review. Int J Radiat Biol 54, 131-50 (1988). WholeCell: PUB_0474, PubMed: 2900272

 
Metadata
Created 2012-10-01 15:08:32
Last updated 2012-10-01 15:16:24